Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Manufacturing of recombinant adeno-associated viral vectors for clinical trials

doi: 10.1038/mtm.2016.2

Figure Lengend Snippet: Institutions and rAAV GMP manufacturing technologies

Article Snippet: Targeted Genetics Corporation, Seattle, WA , wtAd5 Infection , HEK293 Producer HeLa Cell line WAVE and stir Tank bioreactors , Depth filtration, Benzonase, Ion exchange, UF/TFF, Chrom step, Heat inactivation, Chrom step, NanofiltrationPolishingFormulation , 1, 2 , , .

Techniques: Purification, Transfection, Flocculation, Lysis, Filtration, Plasmid Preparation, Concentration Assay, Affinity Chromatography, Sonication, Clarification Assay, Chromatography, Diafiltration Assay, Infection, Ion Exchange Chromatography

Journal: PLoS ONE

Article Title: Loss of Mll3 Catalytic Function Promotes Aberrant Myelopoiesis

doi: 10.1371/journal.pone.0162515

Figure Lengend Snippet: Shown is A , the total number of BM cells, and the percentages of B , B cells and C , Gr1 + Mac1 + myeloid cells, where each point on the graph represents one mouse. Values in panels B and C are expressed as percentages of total cells. D , Representative H&E stains from one individual Mll3 +/+ and Mll3 Δ/Δ mouse of sternum sections taken at 400X magnification. Images depict the entire field of view (top panels) and a zoomed-in view (bottom panels). Scale bars are 50μm. E-I , Similar to panels B and C , except that the percentages of LSK cells, CLP, CMP, MEP, and GMP are shown, respectively. B, C, I , *p<0.05, **p<0.01, ***p<0.005 as determined by the Student’s t -test. G , *p<0.05 as determined by the Mann-Whitney test.

Article Snippet: Control (reference clone) and MLL3 -targeted clones of the human chronic myeloid leukemia cell line KBM7, engineered via a gene-trap system, were purchased from Haplogen (Vienna, Austria) [ ].

Techniques: MANN-WHITNEY

Journal: PLoS ONE

Article Title: Loss of Mll3 Catalytic Function Promotes Aberrant Myelopoiesis

doi: 10.1371/journal.pone.0162515

Figure Lengend Snippet: Absolute gene expression profiling adapted from Gene Expression Commons evaluating Mll3 expression across 39 different hematopoietic cell populations in the mouse BM, spleen, and thymus. Shown are the normalized values of Mll3 expression activity for several cell populations from 2–4 biological replicates. Positive values indicate high Mll3 expression compared to the common reference, and negative values denote relatively low Mll3 expression in comparison to the common reference. Error bars represent mean + SD. HSC, hematopoietic stem cell; Gra, granulocyte; Fr, fraction; Mz, marginal zone; Fo, follicular.

Article Snippet: Control (reference clone) and MLL3 -targeted clones of the human chronic myeloid leukemia cell line KBM7, engineered via a gene-trap system, were purchased from Haplogen (Vienna, Austria) [ ].

Techniques: Gene Expression, Expressing, Activity Assay, Comparison

Journal: PLoS ONE

Article Title: Loss of Mll3 Catalytic Function Promotes Aberrant Myelopoiesis

doi: 10.1371/journal.pone.0162515

Figure Lengend Snippet: The percentage of total cells (top) and absolute number (bottom) of A , B cells, B , T cells, and C , Gr1 + Mac1 + myeloid cells is shown, where each point represents one mouse. D , Representative H&E stains of spleen sections (50X magnification, top panels; 400X magnification, middle panels) from one individual Mll3 +/+ and Mll3 Δ/Δ mouse. Images in the bottom panels are zoomed-in views of the middle panels. Scale bars are 500μm (top panels) and 50μm (middle panels). E , Quantification of splenic follicle size. Each point on the graph represents the average follicle size (black, ≥10 follicles measured; red, <10 follicles measured) for one mouse. *p<0.05, **p<0.01 as determined by the Student’s t -test.

Article Snippet: Control (reference clone) and MLL3 -targeted clones of the human chronic myeloid leukemia cell line KBM7, engineered via a gene-trap system, were purchased from Haplogen (Vienna, Austria) [ ].

Techniques:

Journal: PLoS ONE

Article Title: Loss of Mll3 Catalytic Function Promotes Aberrant Myelopoiesis

doi: 10.1371/journal.pone.0162515

Figure Lengend Snippet: Shown is the percentage of total cells (top) and absolute number (bottom) of A , B cells, B , T cells, and C , Gr1 + Mac1 + myeloid cells. D , Similar to panels A-C , except the total numbers of CD4 + (left) and CD8 + (right) T cells are shown. Each point represents one mouse. B-C , *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 as determined by the Student’s t -test. D , *p<0.05, **p<0.005 as determined by the Mann-Whitney test. E , Representative H&E stains (top panels) and IHC for CD3 (bottom panels) from two individual Mll3 +/+ and Mll3 Δ/Δ mice of LN sections taken at 50X magnification. Scale bars are 500μm.

Article Snippet: Control (reference clone) and MLL3 -targeted clones of the human chronic myeloid leukemia cell line KBM7, engineered via a gene-trap system, were purchased from Haplogen (Vienna, Austria) [ ].

Techniques: MANN-WHITNEY

Journal: PLoS ONE

Article Title: Loss of Mll3 Catalytic Function Promotes Aberrant Myelopoiesis

doi: 10.1371/journal.pone.0162515

Figure Lengend Snippet: A , Relative MLL3 expression in human KBM7 cells as determined by qPCR. TaqMan probes (indicated in red) recognized exon junctions before (exons 18–19) and after (exons 39–40 and 55–56) the gene-trap (exon 38), indicated in green. B , Relative adhesion of KBM7 cells to fibronectin as measured in fluorescence units. Shown is the average of technical replicates from four individual experiments + SEM. C , The number of KBM7 cells that migrated across a trans-well membrane in response to FBS after 24h. Data are the combination of technical replicates from four individual experiments + SD. Ref, control gene-trap; MLL3, MLL3 -targeted gene-trap. *p<0.05, **p<0.01 as determined by the Mann-Whitney test.

Article Snippet: Control (reference clone) and MLL3 -targeted clones of the human chronic myeloid leukemia cell line KBM7, engineered via a gene-trap system, were purchased from Haplogen (Vienna, Austria) [ ].

Techniques: Expressing, Fluorescence, Membrane, Control, MANN-WHITNEY